Axis Shield – Enzymatic Homocysteine

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Project Description

Axis Shield Enzymatic Homocysteine Assay

Order Toll Free: 1-800-267-5800
  • Multiple instrument applications available (Roche, BCI, Siemens and others)
  • Compatible with serum, serum separator and EDTA or heparinized plasma
  • Sample volume from 15µL
  • Limit of detection from 0.8 µmol/L
  • Assay Range up to 46 µmol/L
  • Only 2 calibrators required with calibration stability up to 4 weeks
  • Time to first result from 12 minutes
  • Ready to use, liquid stable reagents and calibrators supplied

Enzymatic Homocysteine

Catalogue # Configuration Product Support
FHRWR 100 MSDS-pdf Product Brochure
FHRWR 1000

Product Information

Intended Use:

The Liquid Stable (LS) 2-Part Enzymatic Homocysteine assay is intended for in vitro quantitative determination of total homocysteine in human serum and plasma.

Assay Principle:

In the Axis-Shield Enzymatic Homocysteine assay, bound or dimerised homocysteine (oxidised form) is reduced to free homocysteine, which then reacts with serine catalysed by cystathionine beta-synthase (CBS) to form L-cystathionine. Cystathionine in turn is broken down by cystathionine beta-lyase (CBL) to form homocysteine, pyruvate and ammonia. Pyruvate is then converted by lactate dehydrogenase (LDH) to lactate with NADH as coenzyme. The rate of NADH conversion to NAD is directly proportional to the concentration of homocysteine.

Homocysteine Control Set
Catalogue # Configuration Product Support
FHCY 200 3 X 1.5mL MSDS-pdf MSDS Sheet