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Pointe Scientific, Inc. has developed and introduced a reagent for the determination of Fructosamine. Fructosamine refers to a protein, which has undergone an Amadori conversion in the presence of glucose to form stable ketoamines. Fructosamine provides a more sensitive assay to the changes in blood glucose control providing earlier detection, a 2-3 week time frame as compared to Glycohemoglobin, which provides a 6-8 week window. This is particularly important during the process of bringing the diabetic patient into control of their glucose levels. The Fructosamine method is based on the ability of ketoamines to reduce NBT to a formazan dye under alkaline conditions. The rate of formazan formation is directly proportional to the Fructosamine concentrations when measured at 550 nm. A comparison study yielded a correlation coefficient of 0.988 and a regression equation of y = 0.88x + 0.28. The reagent kit includes 10 X 11ml, 1 X 120ml buffer (for reconstitution) and a 2.0ml standard. The catalog number is F7546-110. The reagent has18-month shelf life and is linear to 8.5 mmol/L.
Applications are available for a wide range of chemistry analyzers.
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