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Pointe Scientific, Inc. has developed and introduced a reagent for the determination of ß-Hydroxybutyrate. ß-Hydroxybutyrate is a ketoacid present in greatest amounts in serum. In diabetics, the measurement of ß-Hydroxybutyrate as well as blood glucose are needed for the assessment of the severity of diabetic coma and is essential for the exclusion of hyperosmolar non-ketotic diabetic coma. Insulin requirements are often based on the extent of the existing hyperketonemia shown by the blood levels of ß-Hydroxybutyrate. In the Pointe Scientific, Inc. ß-Hydroxybutyrate method, ß - Hydroxybutyrate in the presence of NAD gets converted to acetoacetate and NADH at a pH 8.5 by ß - Hydroxybutyrate dehydrogenase. At this pH, the reaction is favored to the right. The NADH produced is converted to color using INT and diaphorase and read at 505 nm. The reagent kit configuration consist of, 1 X 50 ml R1 and 1 X 8 ml R2, and a 3.0 ml standard. The catalog number is H7587-58. Also available is a three level liquid stable control set with a kit configuration of 6 X 5 mls. The catalog number for the control set is H7587-CTL. The controls have a 60 day open vial stability when stored at 2-8 °C. The assay is linear to 4.5 mmol ß - Hydroxybutyrate.
Applications are available for a number of chemistry analyzers.
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